Preparation, characterization, and functions of rabbit lymph node cell populations

摘要: Complement receptor lymphocytes (CRL) were isolated from lymph nodes of rabbits immunized 6 days earlier with keyhole limpet hemocyanin. Isolation was accomplished by rosetting sheep erythrocytes sensitized antibody and horse complement (EAC) followed Centrifugation on Ficoll-Hypaque (F-H). The CRL population comprised about 30% the starting LNC highly enriched for surface immunoglobulin (SIg) bearing cells. By criterion [3H]thymidine incorporation, responded poorly to Concanavalin A (ConA), but extensively goat anti-rabbit Fab′ (AFab′) ; indeed, better than unfractionated AFab′. Upon addition KLH, produced much less total Ig LNC. KLH-challenged T cells prepared anti-Fab′ columns also synthesized minimal amounts Ig. Antigenic challenge a mixture 3 5 times more sum individual CRL- cell-mediated syntheses. Moreover, KLH concentration dependency, kinetics, ratio 19S7S responses similar reconstituted plus Thus in all examined parameters indistinguishable Therefore, cell populations can be utilized individually combination study their roles vitro induction regulation anamnestic response KLH.