In vitro studies of the rabbit immune system: II. Functional characterization of rabbit T and B populations separated by adherence to nylon wool or lysis with anti-thymocyte serum and complement.
作者： Doug Redelman , Charles B. Scott , Haynes W. Sheppard , Stewart Sell
DOI: 10.1016/0008-8749(76)90127-1
关键词: Lymphocyte 、 Immunology 、 Thymocyte 、 In vitro 、 Molecular biology 、 Population 、 Cell type 、 Biology 、 Spleen 、 Lysis 、 Immune system
摘要: Abstract Rabbit spleen lymphocytes with functions analogous to murine T and B cells have been separated by treatment goat anti-rabbit thymocyte serum plus complement (ATS + C) or passage over columns of nylon wool (NW). ATS C lysis from sheep erythrocyte (SRBC)-immunized rabbits removes 40–60% the total cells, but 80–100% fully differentiated (PFC) are recovered. Conversely, primed which nonadherent NW contain ~5–20% applied only 0.05–1.0% PFC. Neither cell population is capable producing an anti-SRBC PFC response when cultured individually. However, two types each other act synergistically reconsitute in vitro response. Thus, rabbit contains separable lymphocyte populations equivalent as defined system.
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