Subpopulations of mouse spleen lymphocytes. III. Cellular interactions in the response to concanavalin A.

摘要: The profile of response to concanavalin A (con A) purified mouse T cells was found differ appreciably from that non-fractionated spleen cells, in agreement with results previously published by other investigators. Experiments designed elucidate the reasons underlying these differences have revealed con is determined a complex interplay between several cell types. (a) B contribute overall incorporation thymidine presence A-stimulated cells. However, participate only if are dividing. (b) population 'adherent cells' present spleen, which enhances stimulation low doses but suppresses high mitogen. These adherent include most likely conventional macrophages, probably also 'suppressor cells'. (c) Such T' can be readily detected among peritoneal exudate Addition cultures A. This effect further increased treating T-specific antiserum and complement, i.e. eliminating