In vitro studies of the rabbit immune system. III. T-cell requirement for initiation but not maintenance of anti-sheep erythrocyte plaque-forming cells.

摘要: Abstract The in vitro anti-sheep erythrocyte plaque-forming-cell (anti-SRBC PFC) response of primed rabbit spleen cells can be abrogated by prior treatment with anti-thymocyte serum plus complement (ATS + C). direct addition ATS without C to stimulated cultures also reduces the 60–90%, if antiserum is added before initiation antigen-stimulated PFC production. However, becomes refractory inhibition 48 hr. This loss sensitivity not caused T themselves becoming for following reasons: (i) or at hr causes cytotoxic effects comparable similar treatments 0 hr; (ii) responsiveness incubated SRBC remains sensitive that time. result soluble T-cell factors which have accumulated medium, because number continues increase after treating and reculturing fresh medium We conclude are required initiate production antibody B but maintain later proliferative events.