In Vitro Demonstration of Two Antigenically-Distinct Rabbit Lymphocyte Populations

摘要: Two populations of lymphocytes have been demonstrated in the rabbit by using a goat antiserum directed to thymus cells. When incubated with from peripheral blood IgG fraction this (ATG) completely suppressed lymphocyte transformation initiated phytohemagglutinin (PHA) or concanavalin A (Con A) but did not affect stimulation anti-rabbit Fab. Neither preimmune nor ATG absorbed lymph node cells was capable affecting transformation. The method purification and studies involving order addition, effect on human F(ab′) 2 Fab′ fragments indicate that primary mechanism action is prevent binding Con PHA. appeared be lymphocytotoxic under conditions similar those used for experiments. most straightforward interpretation these data vitro selectively eliminates population susceptible PHA does carrying immunoglobulin determinants. Thus thymus-derived their surface receptors ATG, while other expresses immunoglobulin.