Angiogenic lymphokines of activated T-cell origin.

摘要: Inflammation often is accompanied by neovascularization. This especially evident in the normally avascular cornea, where an angiogenic response occurs with keratitis or during graft rejection. In current experiments, supernatants from cultured lymph node cell populations were incorporated Elvax 40 slow-release polymers and implanted corneal stroma. Separation of whole cells into highly T-enriched macrophage permitted investigation soluble products responsible for angiogenesis. It was found that sepharose-linked concanavalin A adequate stimulus generation activity, as assessed rabbit micropocket assay. Cytoadherence, nylon wool column separation, mitomycin C treatment individual used to demonstrate stimulated T are a source material. The time-course magnitude equivalent normal x-irradiated leukopenic animals. These observations potent lymphokine secreted active assay system. Invest Ophthalmol Vis Sci 24:1595-1601, 1983 growth vessels inflammation has been studied extensively since Schoefl's 1 work on fine structure new vasculature wound healing. An documented wide range inflammatory events including arthritis, 2 delayed hypersensitivity, 34 5 class leukocytes can elicit subject considerable investigation. Fromer Klintworth 6 have implicated polymorphonuclear (PMN) primary activity experiments using rat model. Activated macrophages activated angiogenesis guinea pig 4 also be mitogenic fibroblasts, 7 important repair. Using lymphocyte transfer reaction, Auerbach his colleagues evaluated allogeneic lymphocytes mouse 8 9 corneas, chorioallantoic membrane (CAM), skin. 10 Recent Auerbach's laboratory, Lyt Thy series monoclonal antibodies, effector mice," shown major antigenic event associated I-region alloantigens. 12