DNA synthesizing activity in normal human sperm: Location and characterization of the endogenous reaction
作者： S.S. Witkin , A. Bendich
DOI: 10.1016/0014-4827(77)90239-7
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摘要: Abstract Chromatin-associated complexes have been purified from membrane-free nuclei of normal human sperm heads. They band at a sucrose buoyant density 1.15–1.19 g/ml and contain macromolecular components which catalyze the endogenous synthesis DNA in reaction partially sensitive to ribonuclease pretreatment. The sedimentation rate product, still attached its template, demonstrate that [3H]DNA is associated with high molecular weight RNA, may be serving as both template primer.
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