Identification of human CD4 T-cell epitopes on the VP1 capsid protein of enterovirus 71.

摘要: The identification of human CD4 T-cell epitopes within a protein vaccine candidate is great interest,as it provides better understanding the mechanisms involved in protective immunity and may therefore help design effective vaccines diagnostic tools. entire amino acid sequence VP1 capsid from enterovirus 71 (EV 71) strain 41 was submitted to analysis by ProPred algorithm for potential promiscuous T-cellepitopes. Three regions spanning acids 66-77, 145-159, 247-261 were predicted bind more than 25 HLA-DR alleles. corresponding synthetic peptides (SP1 SP3) then tested their abilities induce proliferation T cells isolated five volunteers screened positive previous EV exposure one 71-negative volunteer. Upon stimulation with either peptide, proliferative responses observed all 71-positive volunteers,indicating presence 71-specific memory cells. amplitude peptide- HLA-DR-dependent, correlated well ProPredpredicted binding efficiencies. Moreover, produced significant levels IL-2 IFN- upon stimulation, indicative differentiation into Th-1-type subset. Among three peptides, SP2 induced highest response cytokine production. SP2-induced could be inhibited anti-major histocompatibility complex (MHC) class II antibody, indicating that represents MHC II-restricted epitope. This study demonstrates can accurately predict 71, useful tool subunit against 71.