GTPase inhibiting mutations activate the α chain of G s and stimulate adenylyl cyclase in human pituitary tumours
作者： Claudia A. Landis , Susan B. Masters , Anna Spada , Ann M. Pace , Henry R. Bourne
DOI: 10.1038/340692A0
关键词:
摘要: A subset of growth hormone-secreting human pituitary tumours carries somatic mutations that inhibit GTPase activity a G protein alpha chain, alpha(s). The resulting activation adenylyl cyclase bypasses the cells' normal requirement for trophic hormone. Amino acids substituted in putative gsp oncogene identify domain alpha-chains required intrinsic ability to hydrolyse GTP. This may serve as built-in counter-part separate GTPase-activating proteins GTP hydrolysis by small GTP-binding such p21ras.
nature.com
harvard.edu
elsevier.com
sci-hub.se 参考文章(35)
S E Neal, J F Eccleston, A Hall, M R Webb, Kinetic analysis of the hydrolysis of GTP by p21N-ras. The basal GTPase mechanism. Journal of Biological Chemistry. ,vol. 263, pp. 19718- 19722 ,(1988) , 10.1016/S0021-9258(19)77694-7
J Moss, M Vaughan, Mechanism of action of choleragen. Evidence for ADP-ribosyltransferase activity with arginine as an acceptor. Journal of Biological Chemistry. ,vol. 252, pp. 2455- 2457 ,(1977) , 10.1016/S0021-9258(17)40578-3
R C Thompson, D B Dix, A M Karim, The reaction of ribosomes with elongation factor Tu.GTP complexes. Aminoacyl-tRNA-independent reactions in the elongation cycle determine the accuracy of protein synthesis. Journal of Biological Chemistry. ,vol. 261, pp. 4868- 4874 ,(1986) , 10.1016/S0021-9258(19)89185-8
D. Cassel, F. Eckstein, M. Lowe, Z. Selinger, Determination of the turn-off reaction for the hormone-activated adenylate cyclase. Journal of Biological Chemistry. ,vol. 254, pp. 9835- 9838 ,(1979) , 10.1016/S0021-9258(19)83592-5
P J Casey, A G Gilman, G protein involvement in receptor-effector coupling. Journal of Biological Chemistry. ,vol. 263, pp. 2577- 2580 ,(1988) , 10.1016/S0021-9258(18)69103-3
C Van Dop, M Tsubokawa, H R Bourne, J Ramachandran, Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin. Journal of Biological Chemistry. ,vol. 259, pp. 696- 698 ,(1984) , 10.1016/S0021-9258(17)43512-5
M Freissmuth, M P Graziano, A G Gilman, Expression of Gsα in Escherichia coli: Purification and properties of two forms of the protein Journal of Biological Chemistry. ,vol. 264, pp. 409- 418 ,(1989) , 10.1016/S0021-9258(17)31273-5
Susan B. Masters, Robert M. Stroud, Henry R. Bourne, Family of G protein α chains: amphipathic analysis and predicted structure of functional domains Protein Engineering. ,vol. 1, pp. 47- 54 ,(1986) , 10.1093/PROTEIN/1.1.47
N. Billestrup, L. W. Swanson, W. Vale, Growth hormone-releasing factor stimulates proliferation of somatotrophs in vitro Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 6854- 6857 ,(1986) , 10.1073/PNAS.83.18.6854
L. Vallar, A. Spada, G. Giannattasio, Altered Gs and adenylate cyclase activity in human GH-secreting pituitary adenomas. Nature. ,vol. 330, pp. 566- 568 ,(1987) , 10.1038/330566A0