Mononuclear cell adherence induces neutrophil chemotactic factor/interleukin-8 gene expression.

摘要: The accumulation of polymorphonuclear cells (PMN) in tissue is an essential element the inflammatory response that important host defense. Adherence to endothelium constitutes first step PMN migration from vascular compartment interstitium. We demonstrate human peripheral blood mononuclear (PBMC) adherent plastic can result expression interleukin-8 (IL-8), a potent chemoattractant and activating cytokine. Northern blot analyses showed PBMC expressed IL-8 steady-state mRNA levels by 30 min, peaked at 8 h, then decreased over next 16 h. In contrast, nonadherent (cultured teflon chambers) less than 25% maximal as compared with PBMC. Adherent PBMC-associated determined immunochemistry, supernatant chemotactic bioactivity, extracellular antigenic paralleled expression. Antigenic bioactive were significantly apparent 4-8 respectively, increased 24 Furthermore, gene was suppressed either concomitant treatment actinomycin-D or cycloheximide, yet specific neutralizing antibodies directed against IL-1 beta tumor necrosis factor (TNF)-alpha failed alter adherence-induced levels. These data support hypothesis adherence signal for production IL-8, may be development through elicitation PMN.