Time Dependent Changes in Progesterone Receptor Expression in Cumulus Cells During Meiotic Resumption of Porcine Oocytes
作者： Masayuki Shimada , Masahide Nishibori
DOI: 10.1274/JMOR.20.113
关键词:
摘要: In this study, to investigate the time dependent changes in progesterone receptor (PR) expression cumulus cells during meiotic resumption of porcine oocytes, each amount PR-A and PR-B mRNA was analyzed by RT-PCR with primer sets for region PR-A/B common region. The results showed that levels both were very low immediately recovered from their follicles. cultivation FSH LH significantly increased level PR- B cell COCs, whereas decreased at 12-hr cultivation. Nevertheless, higher maintained up 20-hr cultivation, suggesting mainly expressed 12 hr 20 hr. When COCs cultured 10 then further RU486 hr, proportion oocytes undergoing GVBD a dose fashion. These suggest high ratios are required cumulus-
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