Identification of a Coordinate Regulator of Interleukins 4, 13, and 5 by Cross-Species Sequence Comparisons
作者： Gabriela G Loots , Richard M Locksley , Cathy M Blankespoor , Zhi-En Wang , Webb Miller
DOI: 10.1126/SCIENCE.288.5463.136
关键词:
摘要: Long-range regulatory elements are difficult to discover experimentally; however, they tend be conserved among mammals, suggesting that cross-species sequence comparisons should identify them. To search for sequences, we examined about 1 megabase of orthologous human and mouse sequences noncoding with greater than or equal 70% identity over at least 100 base pairs. Ninety meeting these criteria were discovered, the analysis 15 found across mammals. Characterization largest element in yeast artificial chromosome transgenic mice revealed it a coordinate regulator three genes, interleukin-4,interleukin-13, interleukin-5, spread 120 kilobases.
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