New competitive enzyme-linked immunosorbent assay for determination of metallothionein in tissue and sera.
作者： Margarita Apostolova , Choudomir Nachev , Milena Koleva , Panayot R Bontchev , Ivan Kehaiov
DOI: 10.1016/S0039-9140(97)00341-X
关键词:
摘要: Very little information is available concerning the relationship between metallothionein (MT) and diseases in humans. Several methods to measure MT levels exist but many of these assays are not sensitive human sera. A new competitive ELISA system has been developed using labeled with horseradish peroxidase as a conjugate high-titre polyclonal antibodies obtained from rabbit immunoglobulin G for determination The cELISA proposed here permits reliable range 10-200000 pg ml -1 . method was compared Cd-hem assay showed good agreement results. recovery determined by spiking rat into sera, comparing it spiked diluent controls. overall recoveries added were 101% sera 89% variation within-assay 3 6% respectively. significant difference (P < 0.001) found MT-level patient essential hypertension (646 + 223 ng = 90) normotensive subjects (21 18 236). correlation arterial seems possible. very presented tissues. It enables investigation possible correlations MT-concentration certain diseases.
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