Changes in expression and localization of X-linked inhibitor of apoptosis protein (XIAP) in follicular granulosa cells during atresia in porcine ovaries.

摘要: Follicular selection predominantly depends on granulosa cell apoptosis in porcine ovaries, but the molecular mechanisms regulating induction of cells during follicular remain incompletely understood. To determine role X-linked inhibitor protein (XIAP), which suppresses caspase-3, -7 and -9 activities acts as an endogenous apoptotic death, regulation atresia, we examined changes expression level localization XIAP mRNA atresia using reverse transcription-polymerase chain reaction (RT-PCR), situ hybridization, Western blotting immunohistochemistry, respectively. High levels were noted healthy follicles, decreased atresia. In hybridization immunohistochemistry demonstrated that strongly expressed negative/trace stainings those atretic follicles. The present findings indicate is a candidate molecule anti-apoptotic/pro-survival factor by inhibiting intracellular signaling involved cells.