Changes in expression of interleukin-6 receptors in granulosa cells during follicular atresia in pig ovaries.
作者： Akihisa MAEDA , Yasufumi GOTO , Fuko MATSUDA-MINEHATA , Yuan CHENG , Naoko INOUE
DOI: 10.1262/JRD.19011
关键词: cDNA library 、 Receptor 、 Glycoprotein 130 、 Andrology 、 Biology 、 Follicular phase 、 Granulosa cell 、 Follicular fluid 、 Follicular atresia 、 Atresia 、 Endocrinology 、 Internal medicine
摘要: More than 99% of follicles undergo a degenerative process known as "atresia" in mammalian ovaries, and only few ovulate during follicular growth development. Follicular selection predominantly depends on granulosa cell apoptosis. To reveal the molecular mechanisms selective atresia, we examined changes levels interleukin-6 (IL-6) receptors expressed cells pig ovaries. The IL-6 receptor (IL-6R)-alpha mRNA protein were quantified by reverse transcription-polymerase chain reaction (RT-PCR) Western blotting, respectively. IL-6R alpha highly progressed atretic follicles. Enzyme-linked immunosorbent assay showed that expression soluble (IL-6sR) fluid decreased atresia. Moreover, isolated porcine cDNA encoding an signal transducer, gp130. Porcine gp130 (2,754 bp 917 amino acids) was identified from library prepared using homologous with human murine RT-PCR analysis revealed level also We presume IL-6sR gp130, but not alpha, play important roles regulation survival.
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