Ca2+/Mg2+-dependent endonuclease but not Ca 2+-dependent, Mg2+-dependent or cation-independent endonuclease is involved in granulosa cell apoptosis of pig atretic follicles
作者： Noboru MANABE , Yuzuru IMAI , Yoshihiro KIMURA , Akira MYOUMOTO , Miki SUGIMOTO
DOI: 10.1262/JRD.42.247
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摘要: The antral follicles, 4-6 mm in diameter, were dissected from the ovaries of mature pigs, and then granulosa cumulus cells isolated each follicle. In atretic high activity neutral Ca2+/Mg2+-dependent endonuclease by which chromatin DNA is degraded at internucleosomal sites undergoing apoptosis was noted only but not cells. Low observed both types obtained healthy follicles. activities Ca2+-dependent endonuclease, Mg2+-dependent acidic cation-independent demonstrated all cases. A good correlation (r=0.892) between progesterone/estradiol-17β ratio follicular fluid follicle, provides an index atresia pig ovaries, found. These results suggest that or involved cell follicles pigs.
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