作者: N. Manabe , Y. Imai , H. Ohno , Y. Takahagi , M. Sugimoto
DOI: 10.1007/BF01925566
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摘要: The porcine antral follicles, 3–6 mm in diameter, were dissected from the ovaries of mature pigs, and then granulosa cumulus cells isolated each follicle. In atretic high activity neutral Ca2+/Mg2+-dependent endonuclease DNA ladder formation, estimated by electrophoresis, noted but not cells. Extremely low no formation observed both types obtained healthy follicles. Moreover, apoptotic histochemically among only. A good correlation (r=0.987) between progesterone/estradiol ratio follicular fluid follicle was found. These results suggest that apoptosis occurs follicles pigs.