The role of interleukin-6 in the regulation of granulosa cell apoptosis during follicular atresia in pig ovaries.

摘要: More than 99% of follicles in mammalian ovaries undergo a degenerative process known as atresia, and only few actually ovulate during follicular growth development. Follicular selection mostly depends on granulosa cell apoptosis, but the molecular mechanism behind regulation this selective atresia is still largely unknown. In present study, to examine whether or not interleukin-6 (IL-6), multifunctional cytokine, involved apoptosis pig ovaries, expression IL-6 mRNA cells was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR). The level decreased atresia. Enzyme-linked immunosorbent assay (ELISA) showed that protein fluid also Moreover, recombinant human upregulated an intracellular inhibitor, cellular FLICE-like inhibitory long form (cFLIP(L)), cultured derived from cells. It possible produced healthy follicles, it increases cFLIP(L) level, then prevents apoptotic death.