Quantifying residual HIV-1 replication in patients receiving combination antiretroviral therapy.

摘要: Background In patients infected with human immunodeficiency virus type 1 (HIV-1), combination antiretroviral therapy can result in sustained suppression of plasma levels the virus. However, replication-competent still be recovered from latently resting memory CD4 lymphocytes; this finding raises serious doubts about whether antiviral treatment eradicate HIV-1. Methods We looked for evidence residual HIV-1 replication eight who began soon after infection and whom RNA were undetectable two to three years therapy. examined there had been changes over time proviral sequences peripheral-blood mononuclear cells, which would indicate viral replication. also performed situ hybridization studies on tissues one patient identify cells actively expressing RNA. estimated rate decrease latent, lymphocytes b...