Effects of Altered Nucleotide Concentrations on the Fidelity of DNA Replication

摘要: Until recently, studies on the molecular basis of mutageneis have focused primarily changes caused by mutagenesis that modify DNA. In addition, limited efforts been directed at asking whether modification DNA-replicating proteins affects accuracy DNA replication. Recently there has a growing realization deoxynucleoside triphosphate pools themselves also might influence One most direct approaches to problem is study effects variations in concentrations triphosphates frequency vitro misincorporation purified polymerases. The concept errors made point replication these enzymes are rate-limiting with respect mutagenesis, and misincorporated nucleotides not excised complete efficiency. this manuscript we will review fidelity synthesis as function relative concentration nucleotides. We consider recent suggesting DNA-damaging agents alter nucleotide metabolism cells.