Suppression of TNF-alpha mediated apoptosis by EGF in TNF-alpha sensitive human cervical carcinoma cell line.
作者： Hakan Akca , Selma Yenisoy Akan , Atila Yanikoglu , Osman Nidai Ozes
DOI: 10.1080/0897719031000115369
关键词:
摘要: The tumor suppressor protein p53 is the most frequently mutated gene in human cancer. function of not restricted to "guarding" against oncogenic stress, but also can guard presence DNA damage. One principal mechanisms by which cells achieve this regulating level although its phosphorylation and cellular localization contribute regulation function. Since many tumors secrete growth factor(s) that inhibit apoptosis support cancer cells, we investigated effects epidermal factor (EGF) on TNF-alpha-mediated induction transcriptional target, p21 TNF-alpha sensitive cervical carcinoma cell line, ME180S. We found increase levels p53, induce ME180S cells. However, pretreatment with EGF suppress all these TNF-alpha. To determine kinase(s) pathway was utilized show suppressive effects, were pretreated inhibitors MAPK, PI3K PKC pathways. Among only inhibitor reversed EGF. express zeta, lambda, epsilon, iota, delta, theta, beta subtypes among treatment activate PKC-delta redistribution membrane from cytosol. An PKC, GF 109203X inhibited EGF-mediated suppression TNF-alpha-induced accumulation apoptosis. In summary, concluded protect through activation PKC-delta.
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