Quantification of the major brown shrimp allergen Pen a 1 (tropomyosin) by a monoclonal antibody-based sandwich ELISA

摘要: Abstract Background: Among 13 allergens found in extracts of cooked brown shrimp ( Penaeus aztecus) the 36 kd muscle protein tropomyosin has been identified as only major allergen (Pen a 1). Cross-reacting molecules with similar molecular weights were detected other crustacea species such crab, lobster, and crawfish. Because Pen 1 1-like are important allergy, aim this study was to develop monoclonal antibody (mAb)–based sandwich ELISA quantify evaluate levels four commercial shrimp, lobster extracts. Methods: Two 1-specific mAbs different epitope specificities selected. plates coated captured mAb 3.2 incubated samples containing 1. Bound by combination biotinylated 4.9.5 alkaline phosphatase–labeled streptavidin. Results: The optimized could detect concentrations ranging from 4 125 ng/ml. Four demonstrated 40-fold difference (24 920 μg/ml). Crab contained detectable proteins. No reactivity cockroach, house dust mite, oyster, codfish, or peanut detected, which indicates that developed assay is crustacea-specific. Conclusion: A sensitive This will be helpful standardize regard content allergen, 1, cross-reactivities among occupational exposure species. (J Allergy Clin Immunol 1997;100:229-34.)