Deletion of the single UreG urease activation gene in soybean NIL lines: characterization and pleiotropic effects

摘要: The soybean eu3-a mutant (formerly, eu3-e1) lacks all ureolytic activity. Eu3 encodes urease accessory (Ni insertion) protein, UreG. (Glycine max v1.1 Glyma08g08970.1) is the only UreG-encoding gene in genome. Here we show that lesion a 2.4 kb deletion, beginning 50 bp upstream of transcription start, and covering 94 % deduced amino acid sequence UreG, explaining activity-null phenotype eu3-a. We established near-isogenic lines (NILs), versus Eu3, confirmed striking accumulation endogenous urea none greater resistance leaf discs to external urea. However, incubation light alleviated sensitivity apparently by providing C skeletons for urea-released NH3. Hence, urea, exogenous, less toxic than urea-derived plants, however, did not differ seed Ni content, nor pool sizes metabolites generate via arginine. NILs differed patterns nodulation Bradyrhizobium japonicum elkanii. Ureases have been reported be fungitoxic. NIL seedlings were equally sensitive pathogenic Basidiomycete fungus, Rhizoctonia solani. Since accumulates at least some apo-urease, examined specific anti-fungal role second pair, differing presence (Eu1) absence (eu1-a) urease. Again, this pair exhibited equal R. To our knowledge, report test fungal influenced levels intact plants.