Protease and phospholipase activities of Candida spp. isolated from cutaneous candidiasis

摘要: Abstract Background Cases of superficial and invasive mycoses caused by emerging species Candida have been increasingly reported over the last thirty years. The production hydrolytic enzymes plays a central role in fungal infective process. In infections secretion both proteases phospholipases are well-known virulence attributes. Aims To determine protease phospholipase from 58 human clinical isolates obtained individuals with cutaneous candidiasis seen Human Veterinary Diagnostic Mycology Sector Universidade Federal Fluminense (UFF), Brazil, November 2008 to August 2009. Methods Fungal identification was performed using biochemical tests. Proteolytic activity detected on agar plates containing bovine serum albumin, determined egg-yolk plates. Results isolated were parapsilosis (27.59%), famata (18.96%), albicans (15.52%), haemulonii (12.06%), ciferri (8.62%), guilliermondii (6.90%), tropicalis (5.17%) lipolytica (5.17%). All C. produced phospholipase. As regards non-C. species, 53.06% 4.08% able produce phospholipase, respectively. For example, majority (15/16) protease, while 40% (2/5) producers. This study shows, for first time, that strains protease. Conclusions Collectively, our results showed different lesions and/or phospholipases, which multifunctional molecules directly involved infectious process these fungi.