Glycoprotein 5 of porcine reproductive and respiratory syndrome virus strain SD16 inhibits viral replication and causes G2/M cell cycle arrest, but does not induce cellular apoptosis in Marc-145 cells
作者： Yang Mu , Liangliang Li , Beibei Zhang , Baicheng Huang , Jiming Gao
DOI: 10.1016/J.VIROL.2015.05.019
关键词:
摘要: Abstract Cell apoptosis is common after infection with porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV GP5 has been reported to induce cell apoptosis. To further understand the role of in induced apoptosis, we established Marc-145 lines stably expressing full-length GP5, Δ84-96 (aa 84-96 deletion), Δ97-119 97-119 deletion). proliferation, cycle progression, replication these were evaluated. Neither truncated nor cells. However, , but not or a arrest at G2/M phase resulting reduction growth Additionally, inhibited cells through induction IFN-β. These findings suggest that responsible for inducing under experimental conditions; however it other important roles virus/host biology.
sci-hub.se 参考文章(52)
Yan Ming Zhang, Hai Xia Hong, Pei Sun, Hao Xu, Zheng Yuan Su, Immortalization of swine umbilical vein endothelial cells with human telomerase reverse transcriptase. Molecules and Cells. ,vol. 24, pp. 358- 363 ,(2007)
E J Snijder, J J Meulenberg, The molecular biology of arteriviruses Journal of General Virology. ,vol. 79, pp. 961- 979 ,(1998) , 10.1099/0022-1317-79-5-961
Paloma Su�rez, Ultrastructural pathogenesis of the PRRS virus Veterinary Research. ,vol. 31, pp. 47- 55 ,(2000) , 10.1051/VETRES:2000107
Athanassios C. Tsamandas, Stavroula Salakou, Dimitrios Kardamakis, Vassiliki Zolota, Dimitrios Dougenis, Dionysis S. Bonikos, Vassiliki Tzelepi, Theodore Petsas, Panagiotis Papathanasopoulos, Efstratios Apostolakis, Theodore Papapetropoulos, Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis. in Vivo. ,vol. 21, pp. 123- 132 ,(2007)
J. Gómez-Laguna, F. J. Salguero, M. Fernández de Marco, I. Barranco, I. M. Rodríguez-Gómez, M. Quezada, L. Carrasco, Type 2 Porcine Reproductive and Respiratory Syndrome Virus infection mediated apoptosis in B- and T-cell areas in lymphoid organs of experimentally infected pigs. Transboundary and Emerging Diseases. ,vol. 60, pp. 273- 278 ,(2013) , 10.1111/J.1865-1682.2012.01338.X
Taeg Su Kim, David A. Benfield, Raymond R.R. Rowland, Porcine reproductive and respiratory syndrome virus-induced cell death exhibits features consistent with a nontypical form of apoptosis. Virus Research. ,vol. 85, pp. 133- 140 ,(2002) , 10.1016/S0168-1702(02)00029-1
Wai-Hong Wu, Ying Fang, Rachel Farwell, Melissa Steffen-Bien, Raymond R.R Rowland, Jane Christopher-Hennings, Eric A Nelson, A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Virology. ,vol. 287, pp. 183- 191 ,(2001) , 10.1006/VIRO.2001.1034
Peter G.W. Plagemann, GP5 ectodomain epitope of porcine reproductive and respiratory syndrome virus, strain Lelystad virus Virus Research. ,vol. 102, pp. 225- 230 ,(2004) , 10.1016/J.VIRUSRES.2004.01.031
Richard Y ZHAO, Robert T ELDER, Viral infections and cell cycle G2/M regulation Cell Research. ,vol. 15, pp. 143- 149 ,(2005) , 10.1038/SJ.CR.7290279
C. A. Gagnon, G. Lachapelle, Y. Langelier, B. Massie, S. Dea, Adenoviral-expressed GP5 of porcine respiratory and reproductive syndrome virus differs in its cellular maturation from the authentic viral protein but maintains known biological functions. Archives of Virology. ,vol. 148, pp. 951- 972 ,(2003) , 10.1007/S00705-002-0943-Y