HEMATOPOIESIS IN HUMAN FETAL AND EMBRYONIC LIVER - IMMUNOHISTOCHEMICAL DETERMINATION IN B5-FIXED PARAFFIN-EMBEDDED TISSUES

摘要: Paraffin embedded tissue reactive monoclonal antibodies were used to study human embryonal and fetal haemopoiesis, combining optimal morphology with immunohistological determination of haemopoietic cell subtypes their microenvironment. Seven twelve liver specimens studied, having been fixed in B5-fixative paraffin. The different lineages each showed own immunophenotype distribution; intercellular microenvironmental relationships easily determined. Erythroid cells are VIE-G4, LN1, MT1, sometimes partly surrounding a central macrophage. Myelomonocytic react LCA, MB3, LN2, anti-lysozyme, from 14 weeks onwards LN3. Lymphoid show MT2, MB1, MB2, LN2 reactivity. In few cases some scarce My10+ early progenitor seen. An important finding is the extensive MT1-reactivity distributed over all lineages, demonstration immature blast exclusively expressing MT1 antigen. Further studies employing necessary delineate extent distribution possible function Use mAb may contribute elucidation exact nature place development.