摘要: Abstract The lipase B from Candida antarctica (CAL-B) displays high enantioselectivity on a broad range of substrates, making it an accepted biocatalyst for asymmetric organic chemistry. In recent years, number rational and combinatorial protein engineering projects have focused extending tailoring CAL-B’s catalytic physical properties. Beyond generating customized catalysts, these studies helped to elucidate the enzyme’s structure–function relationship illuminate its enantioselectivity. Potential directions future studies, taking into consideration results efforts related lipases, are discussed.
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MICHAEL FREEMANTLE, Chemistry for water Chemical & Engineering News. ,vol. 82, pp. 25- 30 ,(2004) , 10.1021/CEN-V082N029.P025
Jürgen Pleiss, Markus Fischer, Marcus Peiker, Claudia Thiele, Rolf D. Schmid, Lipase engineering database Journal of Molecular Catalysis B: Enzymatic. ,vol. 10, pp. 491- 508 ,(2000) , 10.1016/S1381-1177(00)00092-8
Uwe Theo Bornscheuer, Romas Joseph Kazlauskas, Hydrolases in Organic Synthesis: Regio- and Stereoselective Biotransformations ,(1999)
Jenny Ottosson, Johanna C. Rotticci-Mulder, Didier Rotticci, Karl Hult, Rational design of enantioselective enzymes requires considerations of entropy. Protein Science. ,vol. 10, pp. 1769- 1774 ,(2001) , 10.1110/PS.13501
Erik Henke, J��rgen Pleiss, Uwe T. Bornscheuer, Activity of lipases and esterases towards tertiary alcohols: insights into structure-function relationships. Angewandte Chemie. ,vol. 41, pp. 3211- 3213 ,(2002) , 10.1002/1521-3773(20020902)41:17<3211::AID-ANIE3211>3.0.CO;2-U
Erik Henke, Uwe T. Bornscheuer, Rolf D. Schmid, Jürgen Pleiss, A Molecular Mechanism of Enantiorecognition of Tertiary Alcohols by Carboxylesterases ChemBioChem. ,vol. 4, pp. 485- 493 ,(2003) , 10.1002/CBIC.200200518
Anke Henne, Ruth A. Schmitz, Mechthild B�meke, Gerhard Gottschalk, Rolf Daniel, Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli. Applied and Environmental Microbiology. ,vol. 66, pp. 3113- 3116 ,(2000) , 10.1128/AEM.66.7.3113-3116.2000
Uwe T. Bornscheuer, Cornelius Bessler, Ramisetti Srinivas, Sajja Hari Krishna, Optimizing lipases and related enzymes for efficient application Trends in Biotechnology. ,vol. 20, pp. 433- 437 ,(2002) , 10.1016/S0167-7799(02)02046-2
Christopher A. Voigt, Carlos Martinez, Zhen-Gang Wang, Stephen L. Mayo, Frances H. Arnold, Protein building blocks preserved by recombination. Nature Structural & Molecular Biology. ,vol. 9, pp. 553- 558 ,(2002) , 10.1038/NSB805
Shamkant A. Patkar, Allan Svendsen, Ole Kirk, Ib Groth Clausen, Kim Borch, Effect of mutation in non-consensus sequence Thr-X-Ser-X-Gly of Candida antarctica lipase B on lipase specificity, specific activity and thermostability Journal of Molecular Catalysis B-enzymatic. ,vol. 3, pp. 51- 54 ,(1997) , 10.1016/S1381-1177(96)00036-7