A novel electrochemical DNA biosensor based on poly-(5-amino-2-mercapto-1,3,4-thiadiazole) modified glassy carbon electrode for the determination of nitrofurantoin
作者： Gözde Aydoğdu , Gülendem Günendi , Derya Koyuncu Zeybek , Bülent Zeybek , Şule Pekyardımcı
DOI: 10.1016/J.SNB.2014.02.083
关键词:
摘要: Abstract In this study, poly(5-amino-2-mercapto-1,3,4-thiadiazole) (PAMT) modified glassy carbon electrode (GCE) was fabricated and used for the electrochemical monitoring of interaction between dsDNA nitrofurantoin (NFT) first time. Electrochemical behavior PAMT GCE (GCE/PAMT) investigated by using cyclic voltammetry (CV) impedance spectroscopy (EIS) compared with those bare GCE. The GCE/PAMT/dsDNA prepared adsorption upon deposited binding NFT via differential pulse (DPV) method. decrease in guanine oxidation peak current at +0.82 V as an indicator 0.5 mol L−1 acetate buffer (pH 4.8) containing 0.02 mol L−1 NaCl. Under optimal conditions, currents were linearly proportional to concentrations range 2–25 mg L−1 detection limit found be 0.65 mg L−1. Furthermore, reproducibility, repeatability, stability applicability analysis pharmaceutical dosage forms human serum samples also examined. These results showed that DNA biosensor could sensitive, accurate precise determination NFT–dsDNA interaction.
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