Physiological characterization of the human EndoC-βH1 β-cell line
作者： Ewa Gurgul-Convey , Martin T. Kaminski , Sigurd Lenzen
DOI: 10.1016/J.BBRC.2015.05.072
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摘要: Abstract In the new human EndoC-βH1 β-cell line, a detailed analysis of physiological characteristics was performed. This line expressed all target structures on gene and protein level, which are crucial for function insulin secretion induced by glucose other secretagogues. Glucose influx measurements revealed an excellent uptake capacity β-cells Glut1 Glut2 transporters. A high expression level glucokinase enabled efficient phosphorylation, increasing ATP/ADP ratio along with stimulation in concentration range. The EC50 value 10.3 mM. Mannoheptulose, specific inhibitor, blocked glucose-induced (GSIS). nutrient secretagogues l -leucine 2-ketoisocaproate also stimulated secretion, potentiating effect -glutamine. Kir 6.2 potassium channel blocker glibenclamide Bay K 8644, opener voltage-sensitive Ca2+ significantly potentiated GSIS. Potentiation GSIS IBMX forskolin went strong cAMP generation. conclusion, fully mirrors analogous primary mouse, rat β-cells. Thus, this is very well suited studies.
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sci-hub.se 参考文章(42)
D. L. Cook, C. N. Hales, L. S. Satin, Glucose Suppresses ATP-Inhibited K-Channels in Pancreatic β-Cells Advances in Experimental Medicine and Biology. ,vol. 211, pp. 63- 67 ,(1986) , 10.1007/978-1-4684-5314-0_5
S. J. H. Ashcroft, P. J. Randle, Enzymes of glucose metabolism in normal mouse pancreatic islets Biochemical Journal. ,vol. 119, pp. 5- 15 ,(1970) , 10.1042/BJ1190005
U. Panten, E.v. Kriegstein, W. Poser, J. Schönborn, A. Hasselblatt, Effects of L‐leucine and α‐ketoisocaproic acid upon insulin secretion and metabolism of isolated pancreatic islets FEBS Letters. ,vol. 20, pp. 225- 228 ,(1972) , 10.1016/0014-5793(72)80801-9
Oscar ALCAZAR, Markus TIEDGE, Sigurd LENZEN, Importance of lactate dehydrogenase for the regulation of glycolytic flux and insulin secretion in insulin-producing cells Biochemical Journal. ,vol. 352, pp. 373- 380 ,(2000) , 10.1042/BJ3520373
G. Parnaud, D. Bosco, T. Berney, F. Pattou, J. Kerr-Conte, M. Y. Donath, C. Bruun, T. Mandrup-Poulsen, N. Billestrup, P. A. Halban, Proliferation of sorted human and rat beta cells Diabetologia. ,vol. 51, pp. 91- 100 ,(2007) , 10.1007/S00125-007-0855-1
Anne Jörns, Tanja Arndt, Andreas Meyer zu Vilsendorf, Jürgen Klempnauer, Dirk Wedekind, Hans-Jürgen Hedrich, Lorella Marselli, Piero Marchetti, Nagakatsu Harada, Yutaka Nakaya, Gen-Sheng Wang, Fraser W. Scott, Conny Gysemans, Chantal Mathieu, Sigurd Lenzen, Islet infiltration, cytokine expression and beta cell death in the NOD mouse, BB rat, Komeda rat, LEW.1AR1-iddm rat and humans with type 1 diabetes Diabetologia. ,vol. 57, pp. 512- 521 ,(2014) , 10.1007/S00125-013-3125-4
Simone Baltrusch, Heike Schmitt, Anke Brix, Sara Langer, Sigurd Lenzen, Additive activation of glucokinase by the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and the chemical activator LY2121260 Biochemical Pharmacology. ,vol. 83, pp. 1300- 1306 ,(2012) , 10.1016/J.BCP.2012.01.020
S. Seino, Cell signalling in insulin secretion: the molecular targets of ATP, cAMP and sulfonylurea Diabetologia. ,vol. 55, pp. 2096- 2108 ,(2012) , 10.1007/S00125-012-2562-9
A De Vos, H Heimberg, E Quartier, P Huypens, L Bouwens, D Pipeleers, F Schuit, Human and rat beta cells differ in glucose transporter but not in glucokinase gene expression. Journal of Clinical Investigation. ,vol. 96, pp. 2489- 2495 ,(1995) , 10.1172/JCI118308
M. J. Smelt, M. M. Faas, B. J. de Haan, P. de Vos, Pancreatic Beta-Cell Purification by Altering FAD and NAD(P)H Metabolism Experimental Diabetes Research. ,vol. 2008, pp. 165360- 165360 ,(2008) , 10.1155/2008/165360