Transcription in human fibroblasts permissively infected by human cytomegalovirus strain AD169.
作者： Sherrol H. McDonough , Deborah H. Spector
DOI: 10.1016/0042-6822(83)90061-2
关键词:
摘要: We used cloned subgenomic DNA fragments of human cytomegalovirus strain AD169 as hybridization reagents to analyze the sites of transcription and abundance of viral RNA in permissively infected human embryonic lung cells. RNA extracted from immediate early, early, middle, and late times in the infection was attached to filters and hybridized with excess cloned subgenomic fragments. Each hybridization was performed with an internal standard to allow quantitation of the RNA concentration and standardization for the variation …
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