Dihydropyrimidine dehydrogenase activity and thymidylate synthase level are associated with response to 5-fluorouracil in human colorectal cancer.
作者： YASUMI ARAKI , HIROHARU ISOMOTO , KAZUO SHIROUZU
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摘要: In the recent studies associated with modulation of 5-fluorouracil (5-FU) and development new antifolates, attentions have been focused on expression target enzymes, thymidylate synthase (TS) dihydropyrimidine dehydrogenase (DPD), that affect tumor sensitivity resistance to drugs. order evaluate predictability therapeutic efficacy by intratumoral enzyme activity, we investigated role TS content DPD activity in 5-FU. Surgical specimens were obtained from 51 patients colorectal cancer used measure activity. tissues measured [3H]-FdUMP binding assay radioenzymatic assay, respectively. The 5-FU was determined collagen-gel droplet embedded-drug test (CDDST). did not correlate Dukes' staging. There no correlation between any tumors. Simple linear regression analysis showed neither (r=-O.267, p>0.05) nor (r=-O.277, p> 0.05) tumors had a significant effectiveness independently. Four out 24 patients, highly responsive 5-FU, low levels both TS. high value either or proved respond conclusion, these results demonstrate are factors predicting responsiveness cancer.
sci-hub.st 参考文章(30)
K-U. Hartmann, Charles Heidelberger, Studies on fluorinated pyrimidines. XIII. Inhibition of thymidylate synthetase. Journal of Biological Chemistry. ,vol. 236, pp. 3006- 3013 ,(1961) , 10.1016/S0021-9258(19)76419-9
Jason Yang, S. Nandi, Growth of Cultured Cells Using Collagen as Substrate International Review of Cytology-a Survey of Cell Biology. ,vol. 81, pp. 249- 286 ,(1983) , 10.1016/S0074-7696(08)62340-2
Sungman Cha, M. H. El Kouni, F. N. M. Naguib, Enzymes of Uracil Catabolism in Normal and Neoplastic Human Tissues Cancer Research. ,vol. 45, pp. 5405- 5412 ,(1985)
H J Lenz, C G Leichman, K D Danenberg, P V Danenberg, S Groshen, H Cohen, L Laine, P Crookes, H Silberman, J Baranda, Y Garcia, J Li, L Leichman, Thymidylate synthase mRNA level in adenocarcinoma of the stomach: a predictor for primary tumor response and overall survival. Journal of Clinical Oncology. ,vol. 14, pp. 176- 182 ,(1996) , 10.1200/JCO.1996.14.1.176
Carlo Aschele, Domizia Debernardis, Stefania Casazza, Giovanna Antonelli, Gianni Tunesi, Chiara Baldo, Rita Lionetto, Frank Maley, Alberto Sobrero, Immunohistochemical quantitation of thymidylate synthase expression in colorectal cancer metastases predicts for clinical outcome to fluorouracil- based chemotherapy Journal of Clinical Oncology. ,vol. 17, pp. 1760- 1770 ,(1999) , 10.1200/JCO.1999.17.6.1760
C G Leichman, H J Lenz, L Leichman, K Danenberg, J Baranda, S Groshen, W Boswell, R Metzger, M Tan, P V Danenberg, Quantitation of intratumoral thymidylate synthase expression predicts for disseminated colorectal cancer response and resistance to protracted-infusion fluorouracil and weekly leucovorin. Journal of Clinical Oncology. ,vol. 15, pp. 3223- 3229 ,(1997) , 10.1200/JCO.1997.15.10.3223
M C Etienne, S Chéradame, J L Fischel, P Formento, O Dassonville, N Renée, M Schneider, A Thyss, F Demard, G Milano, Response to fluorouracil therapy in cancer patients: the role of tumoral dihydropyrimidine dehydrogenase activity. Journal of Clinical Oncology. ,vol. 13, pp. 1663- 1670 ,(1995) , 10.1200/JCO.1995.13.7.1663
Peter V. Danenberg, James Drake, Katherine Behan, Kathleen D. Danenberg, Anne Monks, Diana Nguyen, Jean L. Grem, Allyson Parr, Lauren Young, Carmen J. Allegra, Thymidine kinase, thymidylate synthase, and dihydropyrimidine dehydrogenase profiles of cell lines of the National Cancer Institute's Anticancer Drug Screen. Clinical Cancer Research. ,vol. 7, pp. 999- 1009 ,(2001)
B C Pestalozzi, H F Peterson, R D Gelber, A Goldhirsch, B A Gusterson, H Trihia, J Lindtner, H Cortés-Funes, E Simmoncini, M J Byrne, R Golouh, C M Rudenstam, M Castiglione-Gertsch, C J Allegra, P G Johnston, Prognostic importance of thymidylate synthase expression in early breast cancer. Journal of Clinical Oncology. ,vol. 15, pp. 1923- 1931 ,(1997) , 10.1200/JCO.1997.15.5.1923
P G Johnston, E R Fisher, H E Rockette, B Fisher, N Wolmark, J C Drake, B A Chabner, C J Allegra, The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer. Journal of Clinical Oncology. ,vol. 12, pp. 2640- 2647 ,(1994) , 10.1200/JCO.1994.12.12.2640