Effects of partial/asymmetrical filling of micelles and chiral selectors on capillary electrophoresis enantiomeric separation: generation of a gradient.

摘要: The effect of several experimental parameters on enantiomeric separations in micellar capillary electrophoresis (MCE) was studied. A model separation system tested. It composed an acidic phosphate buffer with sodium dodecyl sulfate (SDS) and hydroxypropyl-β-cyclodextrin (HP-β-CD) as the chiral selector. substituted angelicin used a analyte. Changes concentration SDS micelles/SDS monomers presence HP-β-CD their impact enantioselective were investigated. Variation composition electrolytes individual compartments (inlet vial, capillary, outlet vial) affected both migration times resolution enantiomers. Current vs. time dependencies also monitored during separations. mathematical electromigration systems selector present proposed computer simulation to explain observed phenomena confirm generation CD/SDS-micelle gradient under certain conditions. This is reported first attempt complex, dynamic environment CD-SDS-MCE system.