An anti-Ras function of neurofibromatosis type 2 gene product (NF2/Merlin).

摘要: Previously, we have cloned a candidate for the 595-amino acid neurofibromatosis type 2 tumor suppressor called NF2 or Merlin, with striking sequence similarity in its N-terminal half to an F-actin-binding protein family TERM, which includes talin, ezrin, radixin, and moesin (Trofatter, J. A., MacCollin, M. M., Rutter, L., Murrell, R., Duyao, P., Parry, D. Eldridge, Kley, N., Menon, A. G., Pulaski, K., Haase, V. H., Ambrose, C. Munro, D., Bove, C., Haines, Martuza, R. MacDonald, E., Seizinger, B. Short, Buckler, J., Gusella, F. (1993) Cell 72, 791-800). In attempt determine whether serves as if so is involved anti-oncogenicity, both full-length (NF2-N, residues 9-359) been expressed v-Ha-Ras-transformed NIH/3T3 cells. Like 1 (NF1) fragments (Nur-E-Kamal, S. Varga, Maruta, H. Biol. Chem. 268, 22331-22337), can reverse Ras-induced malignant phenotype, i.e. anchorage-independent growth soft agar, restore contact inhibition of cell growth, indicating that indeed suppressor. Furthermore, NF2-N also suppresses although it appears be less effective than NF2. These observations indicate anti-Ras function resides part half. Thus, new member actin-cytoskeleton-associated proteins, vinculin, alpha-actinin, tropomyosin-1, gelsolin, tensin.