RAGE Mediates the Pro-Migratory Response of Extracellular S100A4 in Human Thyroid Cancer Cells.

摘要: Background: Expression of the small calcium-binding protein S100A4 is associated with poor prognosis in patients thyroid cancer (TC). The authors have previously shown that a target for relaxin and insulin-like peptide 3 signaling TC cells secreted from human cells. Although pro-migratory role intracellular binding to non-muscle myosin well known, this study investigated here whether extracellular contributes migration. Methods: Human cell lines follicular, papillary, undifferentiated cancer, primary patient cells, tissues were utilized discover presence receptor advanced glycation end products (RAGE) tissues. Fluorescence imaging, pull-down assays, Western blot, siRNA silencing, GTPase inhibitors, proliferation, migration assays used investigate interaction RAGE promoting migratory respons...