Real-Time Detection of Telomerase in a Microelectromechanical Systems Platform

摘要: Abstract : The integration of biology with Microelectromechanical Systems and Nanotechnology could result in a synergism tremendous benefits both basic research clinical assays. development functionalized liquid core waveguide to detect biological function or substance via fluorescence have broad range applications. One such target interest for developing system, is the ribonucleoprotein enzyme telomerase. It confirmed biomarker cancer; having been associated over 90% cancers examined. Development MEMS-based detection system would allow accurate small numbers molecules sample volumes. In order accomplish this I first developed bench-top assay identify key components reaction optimize scheme before incorporation into MEMS device. This itself will utility labs telomerase detection. My ongoing efforts synthesize has resulted modification inside surface capillary using rapid, straightforward chemistry indirectly attach molecular beacon. beacon, component capillary, strand DNA that folded specific structure corresponding capture sequence also contains fluorophore quencher moiety. When are close proximity, resonance energy transfer FRET) takes place; effectively quenching flurescence emission. However, binding DNA, RNA, protein beacon causes conformational change separates at which point fluorescent signal can be detected. PDMS chip facilitate fluid control optic fiber couplings