The response of tenocytes to commercial scaffolds used for rotator cuff repair.

摘要: Surgical repairs of rotator cuff tears have high re-tear rates and many scaffolds been developed to augment the repair. Understanding interaction between patients' cells is important for improving scaffold performance tendon healing. In this in vitro study, we investigated response patient-derived tenocytes eight different scaffolds. Tested included X-Repair, Poly-Tape, LARS Ligament, BioFiber (synthetic scaffolds), BioFiber-CM (biosynthetic scaffold), GraftJacket, Permacol, Conexa (biological scaffolds). Cell attachment, proliferation, gene expression, morphology were assessed. After one day, more attached synthetic with dense, fine aligned fibres (X-Repair Poly-Tape). Despite low initial cell human dermal (GraftJacket) promoted greatest proliferation over 13 days. Expression collagen types I III upregulated grown on non-cross-linked porcine dermis (Conexa). Interestingly, ratio mRNA was lower all compared biosynthetic These findings demonstrate significant differences that are routinely used surgery. Synthetic increased adhesion a tendon-like cellular phenotype, while biological expression genes. However, no single superior. Our results may help understand way interact guide development new future.