Proteome analyses of cellular proteins in methicillin-resistant Staphylococcus aureus treated with rhodomyrtone, a novel antibiotic candidate.

摘要: The ethanolic extract from Rhodomyrtus tomentosa leaf exhibited good antibacterial activities against both methicillin-resistant Staphylococcus aureus (MRSA) and S. ATCC 29213. Its minimal inhibitory concentration (MIC) values ranged 31.25–62.5 µg/ml, the bactericidal (MBC) was 250 µg/ml. Rhodomyrtone, an acylphloroglucinol derivative, 62.5–125 times more potent at inhibiting bacteria than extract, MIC MBC were 0.5 µg/ml 2 respectively. To provide insights into mechanisms involved, effects of rhodomyrtone on cellular protein expression MRSA have been investigated using proteomic approaches. Proteome analyses revealed that subinhibitory (0.174 µg/ml) affected several major functional classes whole cell proteins in MRSA. identified involve wall biosynthesis division, degradation, stress response oxidative stress, surface antigen virulence factor, various metabolic pathways such as amino acid, carbohydrate, energy, lipid, nucleotide metabolism. Transmission electron micrographs confirmed morphological ultrastructural alterations treated bacterial cells. Biological processes division interrupted. Prominent changes including wall, abnormal septum formation, disintegration, lysis observed. Unusual size shape staphylococcal cells obviously noted These pioneer findings profiling phenotypic features rhodomyrtone-treated may resolve its antimicrobial which could lead to development a new effective regimen for treatment infections.