Responses in the expression of extracellular proteins in methicillin-resistant Staphylococcus aureus treated with rhodomyrtone

摘要: Rhodomyrtone from a medicinal plant species, Rhodomyrtus tomentosa, is challenged effective agent against Gram-positive bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). The present study was undertaken to provide insight into MRSA extracellular protein expression following rhodomyrtone treatment. Secreteomic approach performed on representative clinical isolate exposing subinhibitory concentration (0.174 μg/ml). identified proteins of response treated condition were both suppressed and overexpressed. Staphylococcal antigenic proteins, immunodominant antigen A (IsaA) staphylococcal secretory (SsaA) involved in cell wall hydrolysis downregulated after the results suggested that may interfere with WalK/WalR (YycG/YycF) system. Other enzymes such as lipase precursor another lipase, glycerophosphoryl diester phosphodiesterase, absent. In contrast, cytoplasmic SpoVG glycerol phosphate lipoteichoic acid synthase, ribosomal found sample. Appearance several culture supernatant revealed bacterial biosynthesis disturbed. This finding provides proteomic mapping rhodomytone Extensive investigation required for this natural compound it has great potency an alternative anti-MRSA drug.