Calcification in atherosclerosis. II. Animal studies.

摘要: Early atherosclerotic lesions in human aortas less than five hours postmortem were studied by light microscopy (20 cases) and electron (10 cases), to determine the morphological cytochemical character of calcium deposition lesions. Routine multiple special stains demonstrated (intimal) be deposited as fine grains, ring-shaped droplets or small needle-shaped crystals, medial grains droplets. The deposits frequently associated with PAS-positive basal lamina surrounding smooth muscle cells. In intimal often granular lipid, while this association was much frequent media. Calcium intima generally not closely elastic fibers but media along near fibers. By composed many cells (with without lipid droplets), newly formed fibers, amorphous ground substance, a few collagen fibrils membrane-limited matrix vesicle-like structures, 100-700 nm diameter. Many similar vesicles present between laminae With potassium pyroantimonate technique for demonstrating calcium, reaction products most concentrated within these also mitochondria cells, extracellular mitochondria-like pericellular lamina-like material loosely dispersed interstitial substance. All negative technique. membrane structures cytochemically positive alkaline phosphatase adenosine triphosphatase. These studies suggest that calcification atherosclerosis is related cell degeneration major initial loci are from degraded comparable osteogenic cartilage.