Increase in alkaline phosphatase activity in calvaria cells cultured with diphosphonates.

摘要: 1. Dichloromethanediphosphonate and to a lesser degree 1-hydroxyethane-1,1-diphosphonate, two compounds characterized by P-C-P bond, increased the alkaline phosphatase activity of cultured rat calvaria cells up 30 times in dose-dependent fashion. 2. Both diphosphonates also slightly inhibited protein synthesis these cells. 3. Thymidine, an inhibitor cell division, did not inhibit induction enzyme, indicating that increase enzyme was due formation specific population with high activity. 4. The effect on suppressed addition cycloheximide, synthesis. 5. After subculturing stimulated medium without diphosphonates, fell almost control value. 6. Bovine parathyrin diminished treated dichloromethanediphosphonate; however, at concentration greater diphosphonate-treated than 7. electrophoretic behaviour, heat inactivation, inhibition bromotetramisole or phenylalanine, Km value induced were identical enzyme.