1,25-Dihydroxyvitamin D3 stimulates the alkaline phosphatase activity of osteoblast-like cells.
作者： S.C. Manolagas , D.W. Burton , L.J. Deftos
DOI: 10.1016/S0021-9258(19)68933-7
关键词:
摘要: Rat osteogenic sarcoma cells which have osteoblast properties including the ability to form bone and mineralize were recently found possess specific cytoplasmic receptors for 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). We examined now effect of 1,25(OH)2D3 other vitamin metabolites on alkaline phosphatase such cell lines. in two lines cultured presence 10(-7) M a 3-fold increase intracellular activity 6-fold secreted activity. The cellular response occurred dose-dependent fashion at range 10(-9) M. In third line, does not receptor 1,25(OH)2D3, we could detect stimulation phosphatase. Vitamin D3, 25-dihydroxyvitamin 24,25-dihydroxyvitamin had no was enhanced increased calcium. view postulated role calcification, speculate that stimulatory osteoblast-like indicates direct involvement mineralization.
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