High-level DNA amplifications are common genetic aberrations in B-cell neoplasms.

摘要: Gene amplification is one of the molecular mechanisms resulting in up-regulation gene expression. In non-Hodgkin's lymphomas, such amplifications have been identified rarely. Using comparative genomic hybridization, a technique that has proven to be very sensitive for detection high-level DNA amplifications, we analyzed 108 cases B-cell neoplasms (42 chronic leukemias, 5 mantle cell and 61 aggressive lymphomas). Twenty-four were 13% patients mapped 15 different regions. Regions most frequently amplified bands Xq26-28, 2p23-24, 2p14-16 as well 18q21 (three times each). Amplification several proto-oncogenes cycle control (N-MYC (two cases), BCL2, CCND2, GLI) located within regions was demonstrated by Southern blot analysis or fluorescence situ hybridization interphase nuclei tumor cells. These data demonstrate are much more frequent than previously assumed. The identification highly genes included amplicons provides important information further analyses genetic events involved lymphomagenesis.