Amplification of Multiple Genes from Chromosomal Region 12q13-14 in Human Malignant Gliomas: Preliminary Mapping of the Amplicons Shows Preferential Involvement of CDK4, SAS, and MDM2

摘要: We have investigated 234 tumors of the central nervous system for amplification 9 different loci from 12q13-14 and report that about 15% anaplastic astrocytomas glioblastomas show at this chromosomal region. The genes most frequently amplified were CDK4 SAS (18 19 cases). MDM2 was coamplified with in 11 while one glioblastoma showed only amplification. Some amplicons additionally included GADD153 (9 cases), GLI (6 A2MR (3 anonymous locus D12S8 (2 Either or highest level each individual amplicon these consistently accompanied by strong overexpression. Our results thus suggest CDK4, SAS, as main targets amplification; however, possibility exists all share a common region between CDK4/SAS which might contain more yet unidentified genes.