Measuring erythrocyte deformability with fluorescence, fluid forces, and optical trapping.
作者： Kapil Bambardekar , Aditya K. Dharmadhikari , Jayashree A. Dharmadhikari , Deepak Mathur , Shobhona Sharma
DOI: 10.1117/1.3037342
关键词:
摘要: A laser-based method has been developed for experimentally probing single red blood cell (RBC) buckling and determining RBC membrane rigidity. Our combines a liquid flow cell, fluorescence microscopy, an optical-trap to facilitate simple measurements of the shear modulus properties RBCs, under physiological conditions. The efficacy is illustrated by studying behavior normal Plasmodium-infected effect Plasmodium falciparum-conditioned medium on normal, uninfected cells. method, which quantifies single-RBC deformability, may ease detection hematological disorders.
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