Androgens modulate epidermal growth factor receptor levels in the rat ventral prostate.

摘要: In order to further understand the factors which influence normal or pathologic growth of prostate, we have characterized receptor for epidermal factor (EGF) in rat ventral prostate and studied hormonal regulation this receptor. EGF binds a single class saturable, high affinity binding sites total prostatic homogenate. Scatchard analysis data reveals an apparent dissociation constant (Kd) 0.93 ± 0.08 nM number 4.01 0.24 fmol per mg protein. Among peptides tested, only native can displace bound [125I]EGF. Castration stimulates concentration receptors from 25.5 3.0 43.4 5.4 fmol/100 tissue intact castrated animals, respectively (P < 0.01). Treatment rats with dihydrotestosterone (DHT) inhibits rise induced by orchiectomy, while estradiol, progesterone dopaminergic agonist CB-154, no effect. Combined administration DHT other above-mentioned steroids CB-154 does not modify inhibition androgen animals. When are expressed as changes treatment reverses inhibitory effect castration yields capacity comparable that measured Chronic pure antiandrogen potent LHRH (LHRH-A) alone has significant on tissue, although, secondary reduction weight, is reduced following LHRH-A treatment. agonist, however, administered combination antiandrogen, rises 12.1 1.3 animals 35.8 4.0 receiving combined highest (9 mg/day) dose antiandrogen. This degree stimulation (about 300%) castration. While both weight strongly modulated androgens, smaller magnitude, thus indicating relative stability parameter tissue. The presence specific