Expression and activities of a recombinant basic fibroblast growth factor-saporin fusion protein
作者： D.A. Lappi , W. Ying , I. Barthelemy , D. Martineau , I. Prieto
DOI: 10.1016/S0021-9258(18)99911-4
关键词:
摘要: A fusion protein containing the full-length sequences of mitogen, basic fibroblast growth factor (FGF-2), and ribosome-inactivating protein, saporin (SAP), has been expressed in E. coli. As expected, it binds with high affinity to heparin-Sepharose like FGF-2 can displace binding radiolabeled its receptor. In contrast, only much lower activity than free saporin, although full be generated by proteolytic removal moiety. Cytotoxicity experiments B16-F10 mouse melanoma cells establish that is active as a chemical conjugate against these intact cells. Presumably have ability activate SAP component through an intra-cellular metabolism protein. Because we also show tumor inhibition properties antimetastatic vivo models melanoma, findings support hypothesis FGF-based ligand-mediated cytotoxicity serve target cytotoxic agents vivo.
sci-hub.st 参考文章(53)
S Kasturi, A Kihara, D FitzGerald, I Pastan, Alanine scanning mutagenesis identifies surface amino acids on domain II of Pseudomonas exotoxin required for cytotoxicity, proper folding, and secretion into periplasm. Journal of Biological Chemistry. ,vol. 267, pp. 23427- 23433 ,(1992) , 10.1016/S0021-9258(18)50108-3
F Stirpe, A Gasperi-Campani, L Barbieri, A Falasca, A Abbondanza, W A Stevens, Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort), of Agrostemma githago L. (corn cockle) and of Asparagus officinalis L. (asparagus), and from the latex of Hura crepitans L. (sandbox tree) Biochemical Journal. ,vol. 216, pp. 617- 625 ,(1983) , 10.1042/BJ2160617
Douglas A. Lappi, Fred S. Esch, Luigi Barbieri, Fiorenzo Stirpe, Marco Soria, Characterization of a Saponaria officinalis seed ribosome-inactivating protein: immunoreactivity and sequence homologies. Biochemical and Biophysical Research Communications. ,vol. 129, pp. 934- 942 ,(1985) , 10.1016/0006-291X(85)91981-3
G W Philpott, M G Santoro, B M Jaffe, Inhibition of B-16 melanoma growth in vivo by a synthetic analog of prostaglandin E2. Cancer Research. ,vol. 37, pp. 3774- 3779 ,(1977)
J.C. vanderSpek, J.A. Mindell, A. Finkelstein, J.R. Murphy, Structure/function analysis of the transmembrane domain of DAB389-interleukin-2, an interleukin-2 receptor-targeted fusion toxin. The amphipathic helical region of the transmembrane domain is essential for the efficient delivery of the catalytic domain to the cytosol of target cells. Journal of Biological Chemistry. ,vol. 268, pp. 12077- 12082 ,(1993) , 10.1016/S0021-9258(19)50310-6
I Pastan, D FitzGerald, Pseudomonas exotoxin: chimeric toxins. Journal of Biological Chemistry. ,vol. 264, pp. 15157- 15160 ,(1989) , 10.1016/S0021-9258(19)84801-9
Clay B. Siegall, Stephen Epstein, Edith Speir, Timothy Hla, Reza Forough, Thomas Maciag, David J. Fitzgerald, Ira Pastan, Cytotoxic activity of chimeric proteins composed of acidic fibroblast growth factor and
Pseudomonas
exotoxin on a variety of cell types The FASEB Journal. ,vol. 5, pp. 2843- 2849 ,(1991) , 10.1096/FASEBJ.5.13.1717336
Thomas N. Oeltmann, Arthur E. Frankel, Advances in immunotoxins
1 The FASEB Journal. ,vol. 5, pp. 2334- 2337 ,(1991) , 10.1096/FASEBJ.5.10.1676688
Davol P, Calabresi P, Beitz Jg, Lappi Da, Baird A, Medina M, Clark Jw, Kato J, Frackelton Ar, Antitumor activity of basic fibroblast growth factor-saporin mitotoxin in vitro and in vivo. Cancer Research. ,vol. 52, pp. 227- 230 ,(1992)
H Lorberboum-Galski, R W Kozak, T A Waldmann, P Bailon, D J FitzGerald, I Pastan, Interleukin 2 (IL2) PE40 is cytotoxic to cells displaying either the p55 or p70 subunit of the IL2 receptor. Journal of Biological Chemistry. ,vol. 263, pp. 18650- 18656 ,(1988) , 10.1016/S0021-9258(18)37334-4