Application of hollow fiber liquid phase microextraction coupled with high-performance liquid chromatography for the study of the osthole pharmacokinetics in cerebral ischemia hypoperfusion rat plasma
作者： Jun Zhou , Ping Zeng , Zhao Hui Cheng , Jing Liu , Feng Qiao Wang
DOI: 10.1016/J.JCHROMB.2011.06.022
关键词:
摘要: Abstract A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction has been developed used to quantify the osthole in cerebral ischemia reperfusion rat plasma following oral administration. The analysis was performed by reversed high performance chromatography with fluorescence detection. Extraction conditions such as solvent identity, agitation rate, salt concentration, extraction time, length of were optimized. Under optimized conditions, linear range 5–500 ng mL −1 ( r 2 = 0.9997). limit detection (LOD) 2 ng mL S / N = 3) quantification (LOQ) being 5 ng mL . validated method successfully applied for pharmacokinetic studies from after
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