Inhibition of androgen and oestrogen production by clomiphene citrate in avian theca cells

摘要: Isolated theca cells (2 X 10(5)/ml) were pre-incubated for 1 h in the presence or absence of clomiphene citrate (10(-12)-10(-4) M). Ovine LH (50 ng/ml) was added and incubated an additional 3 h. A 50% inhibition LH-stimulated androstenedione oestrogen production obtained with doses 10(-8) M 2 10(-7) clomiphene, respectively. Furthermore, effect on reversed by washing from before stimulation LH. In subsequent experiments, effects C17-20-lyase aromatase activities examined. Conversion [3H]17-hydroxyprogesterone to inhibited when pretreated 10(-5) M-clomiphene. addition, conversion testosterone a dose-dependent manner occurring at dose 5 10(-6) M. The results show that treatment vitro inhibits androgen inhibitory aromatase. addition widely-accepted hypothalamic-pituitary axis, present findings add further support suggestion exerts direct ovarian steroidogenesis.