Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol

摘要: Abstract The ability of purified Rous sarcoma virus transforming gene product, pp60v-src, to phosphorylate phosphatidylinositol and diacylglycerol was investigated. Phosphatidylinositol phosphorylated form both mono- diphosphorylated derivatives. 1,2-Diacylglycerol phosphatidic acid. These activities showed the same thermolability sensitivity inhibitors as shown by casein kinase activity pp60v-src. In addition, when serum-starved chicken embryo fibroblasts transformed a mutant temperature-sensitive for transformation were shifted from nonpermissive permissive temperature, an increase 50-100% in labeling 4-phosphate, 4,5-bisphosphate, acid observed, compared uninfected cells.