Hyperflav - perspective photosensitizer for PDT: cell studies.
作者： PV Yermak , NF Gamaleia , AS Shalamay , TV Saienko , VV Kholin
DOI:
关键词:
摘要: Background: Application of hypericin (an alkaloid from Hypericum perforatum plants) as photodynamic agent may become the next successful step in therapy malignant tumors. Hyperflav — is a purified extract designed for purpose diagnosis. Aim: Present studies investigated effectiveness vitro application photosensitizer therapy. Methods: activity was assessed phototoxic cell tests on Jurkat, MT-4 and Namalwa leukemic lines. Spectroscopic measurements solutions were performed. Results: aqueous solubility maintained presence polyvinylpyrrolidone with most pronounced at 1:5 (w/w) Hyperflav-PVP ratio. fluorescence spectrum ethanol exhibits two main peaks around 597 647 nm, accordance pure hypericin. Fluorescence solution 604 655 nm indicates decreasing intensity. drug dose range 5 –25 μg/ml light 15 J/cm 2 showed dose-dependent cytotoxicity tested cultures, while dark not observed. Light irradiation samples preincubated resulted 69.9, 76.0 78.3% death respectively. Combined preparation gold nanoparticles low photocytotoxicity (24.2%) comparison alone (99.6%) cells. Conclusion: being solubilized nontoxic media doses that do have toxicity, therefore it meets requirements perspective photosensitizer.
参考文章(24)
Wierrani F, Loew Hg, Kubin A, Jessner G, Kolbabek H, Burner U, How to make hypericin water-soluble. Die Pharmazie. ,vol. 63, pp. 263- 269 ,(2008)
NF Gamaleia, ED Shishko, GA Dolinsky, AB Shcherbakov, AV Usatenko, VV Kholin, None, Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro. Experimental Oncology. ,vol. 32, pp. 44- ,(2010)
B. Dutrillaux, L. Miccoli, S. Oudard, A. Beurdeley-Thomas, M.-F. Poupon, F. Sureau, G. De Pinieux, Light-induced Photoactivation of Hypericin Affects the Energy Metabolism of Human Glioma Cells by Inhibiting Hexokinase Bound to Mitochondria Cancer Research. ,vol. 58, pp. 5777- 5786 ,(1998)
Weibo Cai, Ting Gao, Hao Hong, Jiangtao Sun, Applications of gold nanoparticles in cancer nanotechnology. Nanotechnology, Science and Applications. ,vol. 1, pp. 17- 32 ,(2008) , 10.2147/NSA.S3788
Claudine A Robertson, D Hawkins Evans, Heidi Abrahamse, None, Photodynamic therapy (PDT): a short review on cellular mechanisms and cancer research applications for PDT. Journal of Photochemistry and Photobiology B-biology. ,vol. 96, pp. 1- 8 ,(2009) , 10.1016/J.JPHOTOBIOL.2009.04.001
D. Skalkos, E. Gioti, C.D. Stalikas, H. Meyer, Th.G. Papazoglou, G. Filippidis, Photophysical properties of Hypericum perforatum L. extracts--novel photosensitizers for PDT. Journal of Photochemistry and Photobiology B-biology. ,vol. 82, pp. 146- 151 ,(2006) , 10.1016/J.JPHOTOBIOL.2005.11.001
Roberto Augusto Fernandes Machado, Antonio Marcelo Barbante Casella, Marcus Rudolph Malaguido, Ana Paula Miyagusko Taba Oguido, Estudo experimental da inibição da proliferação vitreorretiniana pelo uso da hiperecina Arquivos Brasileiros De Oftalmologia. ,vol. 72, pp. 650- 654 ,(2009) , 10.1590/S0004-27492009000500010
Emina Besic, Patrick Forny, Daniel Lüscher, Klaus Grätz, Caroline Maake, Heinrich Walt, [5.05] Photodynamic effects of hypericin and Foslipos in head and neck squamous cell carcinoma in vitro Medical Laser Application. ,vol. 24, pp. 136- 137 ,(2009) , 10.1016/J.MLA.2009.02.033
N.E. Stavropoulos, A. Kim, U.U. Nseyo, I. Tsimaris, T.D. Chung, T.A. Miller, M. Redlak, U.O. Nseyo, D. Skalkos, Hypericum perforatum L. extract - novel photosensitizer against human bladder cancer cells. Journal of Photochemistry and Photobiology B-biology. ,vol. 84, pp. 64- 69 ,(2006) , 10.1016/J.JPHOTOBIOL.2006.02.001
Dieter Jocham, Herbert Stepp, Raphaela Waidelich, Photodynamic Diagnosis in Urology: State-of-the-Art European Urology. ,vol. 53, pp. 1138- 1150 ,(2008) , 10.1016/J.EURURO.2007.11.048